Anti-Murine CD31 Antibody Clone SZ31 for standard FFPE tissues

Antibody clone SZ31 is the first antibody which reacts specifically with murine CD31 in standard formalin-fixed paraffin-embedded tissue sections.

CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) is expressed constitutively on the surface of embryonic and adult endothelial cells. It is also expressed on cell surfaces of monocytes, neutrophils, platelets and certain T-cell subsets. It has been detected on bone marrow-derived hematopoetic stem cells and embryonic stem cells. CD31 is a 130kDa integral membrane glycoprotein and as a member of the immunoglobulin superfamily involved in the mediation of cell-to-cell adhesion. CD31-mediated endothelial cell-cell interactions play a major role in angiogenesis. Studies have shown CD31 to be a superior marker in human angiogenesis, which reportedly predicts tumor recurrence.

Pathophysiological studies of CD31 in murine model systems had limitations because standard formalin-fixed sections were excluded. The rat clone SZ31 eliminates these restrictions by allowing high quality immunohistochemical analysis of standard formalin-fixed paraffin sections in mice.

• Productdetails: Clone SZ31 as a "goldstandard" for the analysis of angiogenesis in mouse models

• dianova-antibodies for angiogenesis & cell surface receptors

A-R: Immunohistochemistry of murine CD31 (PECAM-1) in standard formalin-fixed paraffin-embedded tissue sections. All sections were stained by an indirect horseradish peroxidase (HRP)-method according to standard procedures, counterstaining with Hämatoxylin.

The monoclonal antibody clone SZ31 reacts specifically with endothelial cells in vessels and capillaries of the following murine tissues:
A: Aorta
B: Aortic origin
C: Endocardium
D-G: Small intestine
H: Colon
I: Brain
J-L: Lymph nodes
M: Bone marrow
N-O: Mesenterial vessels
P: Kidney
Q-R: Pancreas

(pictures courtesy of Prof. Dr. Robert Klopfleisch, Institute of Pathology, Department of Veterinary Pathology, Berlin, Germany)

A-F Immunohistochemistry of murine CD31 (PECAM-1) in standard formalin-fixed paraffin-embedded tissue sections. All sections were stained by an indirect alkaline phosphatase method according to standard procedures, counterstaining with Hämatoxylin-Papanicolaou.
The monoclonal antibody clone SZ31 reacts specifically with endothelial cells in vessels and capillaries of the following murine tissues:
A: Lung
B: Skeletal muscle
C: Spinal cord
D: Liver
E-F: Adenocarcinoma

 Western blot analysis: Immunoblot of extracts from murine lung, J558L cells and m-Lend cells using CD31 rat monoclonal antibody  clone SZ31 (DIA-310 1:5.000) and goat anti-rat-HRP antibody (1:10.000)

Lane:
1 J558 cells (CD31-), 25µg lysate
2 murine lung, 25µg lysate
3 blank
4 m-Lend cells (CD31+), 12,5µg lysate

(pictures courtesy of Prof. Dr. Harald Stein, Institute of Pathology, Charité Campus Benjamin Franklin, Berlin, Germany)

Instructions for use

Immunohistochemical staining of standard formalin-fixed paraffin sections
Indirect alkaline phosphatase staining (Other techniques, e.g. Avidin-Biotin-alkaline phosphatase (ABAP), alkaline phosphatase anti-alkaline phosphatase (APAAP) or horseradish peroxidase (HRP) -method are also possible).

1. Deparaffinize formalin-fixed paraffin-embedded mouse tissue sections by a standard procedure using xylol/ethanol
2. Antigen retrieval: high temperature heating of sections in citrate buffer pH 6,0 according to standard procedures
3. Block with 5% rabbit serum, 10 min RT
4. Wash with TBS, 3 x 5 min
5. Incubate with antibody clone SZ31 (1:10-1:20), 30min RT
6. Wash with TBS, 3 x 5 min
7. Incubate with rabbit anti-rat IgG (H+L) alkaline phosphatase (1:200), 30min RT
8. Wash with TBS, 3 x 5 min
9. Add substrate, e.g. Neufuchsin, 30min RT
10. Counterstain, e.g. with Hematoxylin-Papanicolaou

References

1. Hölscher M, Silter M, Krull S, von Ahlen M, Hesse A, Schwartz P, Wielockx B, Breier G, Katschinski DM, Zieseniss A. Cardiomyocyte-specific prolyl-4-hydroxylase domain 2 knock out protects from acute myocardial ischemic injury. J.Biol.Chem. 286: 11185-11194, 2011
2. Kröger C, Vijayaraj P, Reuter U, Windoffer R, Simmons D, Heukamp L, Leube R, Magin TM. Plceental vasculogenesis is regulated by keratin-mediated hyperoxia in murine decidual tissues. Am.J.Pathol. 178(4): 1587-1590, 2011