Donkey IgG anti-Sheep IgG (H+L)-Brilliant Violet 480, MinX Ck,Gp,Hs,Ho,Hu,Ms,Rb,Rt
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Overview
SKU 713-685-147 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections)
Conjugation Maximum Absorption Maximum Emission No Cross-reactivity (MinX) with Chicken, Guinea Pig, Hamster syrian, Horse, Human, Mouse, Rabbit, Rat
Dilution Format 0.005% Tween 20, 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 150 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS pH7.2, lyophilisate
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule sheep IgG. It also reacts with the light chains of other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
BD Brilliant Violet 480 (BV480) has an Excitation peak at 436 nm with maximal emission at 478 nm. Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.
If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet 421, Brilliant Violet 480, Alexa Fluor 488 and Rhodamine Red-X. If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet 421, Brilliant Violet 480, Alexa Fluor 488 and Rhodamine Red-X. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5 (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5‘s excitation and emission profiles overlap those of Alexa Fluor 647.