DIA-808 – Anti-CD8a (Ms) from Rat (Clone: GHH8) for mouse FFPE tissue – 200 µl

Art.-Nr.
DIA-808

Specificity
CD8a (MHC I Co-Receptor, cytotox. T-cells)
Species Reactivity
Mouse
Host Species
Rat
Isotype
IgG2a/k
Clone
GHH8
Clonality (Mono-/Polyclonal)
monoclonal
Application
Immunohistochemistry (Paraffin-embedded Sections)
Conjugation
unconjugated
Format
lyophilisate
antibody purified (from culture supernatant)
Product line / Topic
CD markers
immunohistochemistry
Intended Use
for Research Use Only
Temperature – Storage
2-8°C
Search Code
DIA808
Manufacturer / Brand
dianova

Reactivity:

Antibody clone GHH8 has been developed and validated for the detection of murine CD8a in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). Clone GHH8 identifies cytotoxic and regulatory T-cells (also known as suppressor T-cells) in murine FFPE tissue by IHC.

Background:

CD8 is a marker for T-cells with suppressor and cytotoxic activity. Cluster of Differentiation 8 (CD8) is also found on the surface of some natural killer cells, most thymocytes, some T-cell lymphomas and large granular lymphocyte leukemias.
Antigen recognition and subsequent T-cell activation is controlled by the interaction between TCR (T-cell receptor) and pMHC (peptide-major histocompatibility complexe). T-cell activation is often enhanced by a close cooperation of the TCR with two different co-receptors: CD8 and CD4. CD8 was first discovered as a cell surface marker in mice and was used to discriminate CD8+ CTLs and CD4+ Th cells. CD8a is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). CD8 alpha beta is expressed on the majority of cytotoxic T-lymphocytes (CTLs). This CD8 heteromeric proteins (with approx. 32-34 KDa each monomer) are physically associated with a p56 tyrosine kinase to mediate T-cell activation signals through phosphorylation of adjacent proteins.
Besides its important role in T-cell signalling, the CD8 co-receptor is also important for CD8+ T-cell development, stabilisation of the interface between CD8+ T-cells and target cells, and activation of intestinal epithelial T lymphocytes (iIELs). Therefore, CD8 has been the target of many therapeutic strategies designed to modulate T-cell responses during auto-immune reactions, allergy and organ transplant rejection.

Immunohistochemistry of mouse CD8a in formalin-fixed paraffin-embedded tissue sections
Immunohistochemical staining (IHC) with anti-CD8a (MHC I Co-Rezeptor, zytotox. T-Zellen) Antibody (clone GHH8) - dianova

A

Immunohistochemical staining (IHC) with anti-CD8a (MHC I Co-Rezeptor, zytotox. T-Zellen) Antibody (clone GHH8) - dianova

B

A, Mouse spleen: The monoclonal antibody clone GHH8 specifically stains CD8a in standard formalin-fixed paraffin-embedded mouse spleen. Incubation with antibody clone GHH8 (5μg/ml, corresponding to a 1:100 dilution) for 1h at RT. Detection with ImmPRESS HRP anti-Rat from Vector Laboratories (Picture courtesy of Christel Bonnas, Synaptic Systems, Göttingen, Germany).
B, Mouse liver after viral infection: Specific Detection of invading T-cells with monoclonal antibody clone GHH8 by IHC-FFPE. Note the clear membrane-bound signal free of background. Detection with a Ventana automated stainer following standard procedures. Incubation for 30min at 4°C with 10μg/ml of antibody clone GHH8, corresponding to a 1:50 dilution (Picture courtesy of Susanne Krasemann, Mouse Pathology Core Facility, UKE, Hamburg, Germany).

Specific References:

  1. Kuczynski EA, Krueger J, Chow A, Xu P, Man S, Sundaravadanam Y, Miller JK, Krzyzanowski PM, Kerbel RS. Impact of chemical-induced mutational load increase on immune checkpoint therapy in poorly responsive murine tumors. Mol Cancer Ther. 2018 Feb 26; PMID: 29483207
  2. Wüstner S, Anderl F, Wanisch A, Sachs C, Steiger K, Nerlich A, Vieth M, Mejías-Luque R, Gerhard M. Helicobacter pylori γ-glutamyl transferase contributes to colonization and differential recruitment of T cells during persistence. Sci Rep. 2017 Oct 20;7(1):13636. PMCID: PMC5651840

General References:

  1. Kersh AE et al. Cutting Edge: Resident Memory CD8 T Cells Express High-Affinity TCRs. Journal of Immunology 2015, 195: 3520-4.
  2. Wu RC et al. New insights on the role of CD8(+)CD57(+) T-cells in cancer. Oncoimmunology 2012, 1: 954-56.
  3. Hyland L et al. Mice lacking CD8+ T cells develop greater numbers of IgA-producing cells in response to a respiratory virus infection. Virology 1994, 204: 234-41.
  4. Prince HE et al. CD4 and CD8 subsets defined by dual-color cytofluorometry which distinguish symptomatic from asymptomatic blood donors seropositive for human immunodeficiency virus. Diagnostic & Clinical Immunology 1987, 5: 188-93.
  5. Gaudernack G et al. Isolation of pure functionally active CD8+ T cells. Positive selection with monoclonal antibodies directly conjugated to monosized magnetic microspheres. Journal of Immunological Methods 1986, 90: 179-87.
  6. Fleischer B et al. Function of the CD4 and CD8 molecules on human cytotoxic T lymphocytes: regulation of T cell triggering. Journal of Immunology 1986,136: 1625-8.