DIA-SY1-OD – Anti-CD138 (Syndecan-1) (Hu) from Mouse (JASY1) – unconj. – 100 µl

Art.-Nr.
DIA-SY1-OD

Specificity
CD138 (Syndecan-1)
Species Reactivity
Human
Host Species
Mouse
Isotype
IgG1/k
Clone
JASY1
Clonality (Mono-/Polyclonal)
monoclonal
Application
Immunohistochemistry (Paraffin-embedded Sections)
ELISA
Immunohistochemistry (IHC)
Conjugation
unconjugated
Dilution
Immunohistochemistry (IHC): 1:100 – 1:400
Format
ProteinA/G purified antibody
concentrate
1% BSA
in Tris buffer (pH 7.3-7.7)
< 0.1% NaN3
Immunogen
Recombinant Human CD138
Intended Use
for Research Use Only
Temperature – Storage
2-8°C
Temperature – Transport
cooled
Manufacturer / Brand
ONCOdianova
Alias
Syndecan-1
SYND1
CD138
SDC1
SDC
Syndecan Proteoglycan 1
Syndecan 1

Ultra-validated anti-CD138 IHC-Gallery

Clone JASY1 is ultra-validated for IHC detection of CD138 plasma cell marker in routine-fixed FFPE tissue sections.

Oncodianova future Biomarkers

ONCOdianova develops new antibodies for IHC detection of cancer biomarkers in FFPE-tissues.

Reactivity

Mouse monoclonal anti-CD138 antibody clone JASY1 is suitable for the immunohistological detection of CD138 in routine-fixed paraffin embedded tissue sections.
Cluster of differentiation 138 (CD138), also known as Syndecan-1, is a transmembrane glycoprotein, (heparan sulphate proteoglycan) expressed on the surface of plasma cells within the hematopoietic system and on the surface of mature epithelial cells. CD138 is composed of a single chain transmembrane core protein (30,5 KDa, comprising a short cytoplasmic domain, a transmembrane domain, and a long extracellular domain) and five covalently attached glycosaminoglycan.
In diagnostic surgical pathology, antibodies against CD138 are commonly used to identify and quantitate plasma cells. Syndecan-1 (CD138) is a cell surface protein with relevance for cell-cell and cell-matrix interaction. In normal tissues, CD138 is expressed on plasma cells but also in various epithelial cell types. CD138 is also expressed in various cancers. In several tumor types CD138 expression levels were described to be prognostically relevant. CD138 expression in cancer is of potential clinical interest. Antibody-based drugs targeting CD138 in plasmocytomas are being evaluated in clinical trials. In preclinical studies, anti-CD138 antibodies were also effective against triple negative breast cancer and melanoma cells.

Immunohistochemistry of human CD138 in routine formalin-fixed paraffin-embedded (FFPE) tissue samples

A: Basal-cell carcinoma with strong membranous CD138 staining.
B: Colon adenoma with intense predominantly membranous CD138 staining. Numerous CD138 positive plasma cells are located in the stroma.
C: Strong membraneous CD138 staining of hepatocellular carcinoma.
D: Hodgkin lymphoma showing strong CD138 staining of plasma cells.


(A) Basal-cell carcinoma


B) Colon adenoma


(C) Hepatocellular carcinoma


(D) Hodgkin lymphoma

(Pictures kindly provided by Christoph Fraune & Christina Merenkow, Department of Pathology, University Hospital Eppendorf (UKE), Hamburg, Germany)

References

  1. Carbone A, et al. (1998) Differential expression of BCL-6, CD138/syndecan-1, and Epstein-Barr virus-encoded latent membrane protein-1 identifies distinct histogenetic subsets of acquired immunodeficiency syndrome-related non-Hodgkin’s lymphomas. Blood 91: 747-755
  2. Anttonen A et al. (1999) Syndecan-1 expression has prognostic significance in head and neck carcinoma. Br J Can-cer. 79(3-4): 558-564
  3. Chilosi M et al. (1999) CD138/syndecan-1: a useful immunohistochemical marker of normal and neoplastic plasma cells on routine trephine bone marrow biopsies. Mod Pathol. 12: 1101-1106
  4. Sebestyén A et al. (1999) Syndecan-1 (CD138) expression in human non-Hodgkin lymphomas. Br J Haematol. 104: 412-419
  5. Bayer-Garner IB et al. (2001) Syndecan-1 (CD138) immunoreactivity in bone marrow biopsies of multiple myeloma: shed syndecan-1 accumulates in fibrotic regions. Mod Pathol. 14: 1052-1058
  6. O’Connell FP et al. (2004) CD138 (syndecan-1), a plasma cell marker immunohistochemical profile in hematopoietic and nonhematopoietic neoplasms. Am J Clin Pathol. 121: 254-63.
  7. Sanderson RD et al. (2008) Syndecan-1: a dynamic regulator of the myeloma microenvironment. Clin Exp Metasta-sis. 25: 149-159
  8. Colomo L et al. (2004) Diffuse large B-cell lymphomas with plasmablastic differentiation represent a heterogeneous group of disease entities. Am J Surg Pathol. 28: 736-747

Quantity: 100 µl

Usual delivery time: 10 business days


328,00  plus VAT
Free shipping inside Germany