Mouse IgG anti-Sheep IgG (L) (9A12-2E10)-Biotin, MinX Ho,Hu,Ms,Rb,Rt
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Overview
SKU 213-062-177 Host Species IgG Form Species Reactivity Specificity Isotype Clone Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Western Blot
Conjugation No Cross-reactivity (MinX) with Dilution ELISA 1:20,000 – 1:400,000, Flow Cytometry 1:200 – 1:1,000, Immunofluorescence 1:200 – 1:1,000, Immunohistochemistry (IHC): 1:500 – 1:5,000, Western Blot (WB): 1:20,000 – 1:400,000
Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on sheep IgG and with those common to other sheep immunoglobulins. The antibody does not react with the heavy chain of sheep IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, horse, human, mouse, rabbit, and rat immunoglobulins, but it may cross-react with immunoglobulins from other species.
Conjugate
Biotin-SP is the trade name of Jackson ImmunoResearch for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.