Donkey F(ab’)2 anti-Human IgM (µ)-Alk. Phos., MinX Bo,Ho
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Overview
SKU 709-056-073 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Western Blot
Conjugation No Cross-reactivity (MinX) with Dilution ELISA 1:5,000 – 1:50,000, Histo-/Cytochemistry 1:500 – 1:5,000, Western Blot (WB): 1:5,000 – 1:50,000
Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM Tris-HCI (pH 8.0), lyophilisate
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine and horse serum proteins, but it may cross-react with IgM from other species.
Conjugate
Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.