Alpaca Secondary Antibodies

We extended our portfolio of immunoglobulins for the life science research community with the new alpaca (Vicugna pacos) secondary antibodies. This host species is for example suitable for multiple labeling experiments, when antibodies from donkey or goat are not the right choice because of cross-reactivity. Alpaca together with Llama belong to the family of Camelidae. This species have a unique class of immunoglobulins among mammals. Three IgG-subclasses are found in their sera: the conventional IgG1 as well as IgG2 and IgG3. The latter lack the light chain and first-constant (CH1) domain (Fig.1). These heavy chain-only antibodies are smaller than normal IgGs, but are robust and have full specificity and affinity to their respective antigens.

The polyclonal alpaca secondary antibodies (complete set of IgG1, IgG2 and IgG3) from Jackson ImmunoResearch detect Rabbit IgG (H+L), Mouse IgG (H+L) or Human IgG (H+L). To avoid any unspecific background staining due to BSA blocking, all antibodies are cross-adsorbed against bovine serum proteins. In addition, they have no cross-reactivity with rabbit, mouse or human, depending on your choice of species-reactivity.

Scheme of Alpaca IgG-subclasses
Fig.1: Scheme of conventionel IgG (left), and heavy chain-only Alpaca IgG2/ IgG3 (right).

Secondary Antibodies from Alpaca are available with the following conjugates:

• Unconjugated
• Horseradish Peroxidase
• Alkaline Phosphatase
• Biotin
• DyLight™ 405
• AMCA
• Cy™ 2
• Alexa Fluor® 488
• Fluorescein (FITC)
• Cy™ 3
• Rhodamine (TRITC)
• Alexa Fluor® 594
• Rhodamine Red-X™
• Alexa Fluor® 647
• Cy™ 5

References:

  • Hamers-Casterman, C., T. Atarhouch, S. Muyldermans, G. Robinson, C. Hamers, E.B. Songa, N. Bendahman, and R. Hamers. 1993. Naturally occurring antibodies devoid of light chains. Nature. 363:446–448
  • De Genst E, Saerens D, Muyldermans S, Conrath K. Antibody repertoire development in camelids. Dev Comp Immunol (2006) 30:187–98.10.1016/j.dci.2005.06.010
  • Maass DR, Sepulveda J, Pernthaner A, Shoemaker CB. Alpaca (Lama pacos) as a convenient source of recombinant camelid heavy chain antibodies (VHHs). J Immunol Methods (2007) 324:13–25.10.1016/j.jim.2007.04.008