High affinity His-Tag antibody from dianova
Highest Affinity & Sensivity: Biacore 3×10-10M
The mouse monoclonal antibody clone 13/45/31-2 (Isotype IgG1) detects N- and C-terminally His-tagged proteines as well as internal His-tags. With a high affinity of 3x 10-10M (BiacoreTM) it is optimally suited for a large variety of applications.
The anti-His-epitope-tag clone 13/45/31/2 has been developed for optimal detection in cells and complex cell-lysates (Prof. Zentgraf, DKFZ, Heidelberg, Germany). Clone 13/45/31-2 shows a very high specificity and sensitivity against recombinant proteins with a tag of at least 6 histidines. Constructs with 4 additional histidines show 20- to 40-times increased affinity without any significant impact on the tertiary structur of the expressed protein.
Binding of the epitope-tag antibody is not influenced by the flanking aa-sequence of the constructs offering a broad choice in the selection of available expression vectors. The only requirement for antibody binding is the stereoscopic accessibility of the epitope
Application Chart His-Epitope-Tag Antibody
|EM||Elektron mikroskopy||Assay dependent|
|FACS – Flow||Flow Cytometry||1:500-1:2000|
|IHC-F||Immunohistochemistry – Frozen||Assay dependent|
|IHC-P / FFPE||Immunohistochemistry – Paraffin||Assay dependent|
|WB (colorimetric)||Western Blot (colorimetric)||1:100 – 1:10.000|
|WB (ECL)||Western Blot (ECL)||1:1.000 – 1:10.000|