LigaTrap™ Antibody Purification

For many years Protein A and Protein G Chromatography are the standard tools for antibody purification. LigaTrap™ Technologies provides a novel and patented portfolio of affinity ligands, which are developed for monoclonal and polyclonal immunoglobulins purification. In addition to species-specific ligands (Human IgM, Mouse IgG (all Isotypes), Chicken IgY and more), a distinct advantage is the ability to elute bound antibodies at a milder pH (pH 4.0) than commonly used protein G and A resins (pH ≤ 3.0). This reduces the risk of precipitation and inactivation of pH-sensitive antibodies. After few simple steps (loading the columns, washing, elution) you recover a high yield of pure product with LigaTrap™ ready-to-use Kits for antibody purification. For antibody purification we also provide loose IgG-specific Resin as well as prepacked columns.

Purification of Human IgM

IgM monoclonal antibodies are popular among the research and diagnostic community, while gaining more interest as potential therapeutic candidate. However, most ligands for antibody purification, such as Protein A and Protein G Chromatography, are not suitable for all immunoglobulin subclasses. Therefore, IgM is often referred to as “the forgotten immunoglobulin”. LigaTrap™ products are the first rapid and cost-effective way to specifically purify human IgM antibodies. Find out about the benefits of IgM purification with LigaTrap™ products in this Whitepaper.

Learn more about LigaTrap™ Human / Mouse IgM Purification.

Human IgM Purification - LigaTrap LT-143

IgY: Purification from Chicken Serum

Immunoglobulin Y (IgY) is isolated from serum or eggs of chicken, but high concentrations of lipids in egg yolk can interfer with antibody purification. In addition, standard purification plattforms (Protein A and G Chromatography) do not bind IgY. With LigaTrap™ Chicken IgY Purification Kit we provide a robust, quick and simple method to purify IgY from chicken serum.

Learn more about the Chicken IgY Isolationkit LT-142Kit.

Chicken IgY Purification - LigaTrap

IgG1, IgG2 and IgG3: Purification from Llama Serum

Llama (Lama glama) along with Alpaca and Guanaco belong to the familiy Camelidae. These animals have a unique class of immunoglobulins among mammals: Next to IgG1, with its classical immunoglobulin-structur (two light chains and two heavy chains), heavy chain-only IgGs circulate in their blood. IgG2 and IgG3 lack any light chain and are smaller (94 kDa, Llama) than normal IgG1 antibodies (150 kDa). LigaTrap™ Llama IgG Resins bind and capture all three isotypes.

LigaTrap Llama IgG scheme

Prepacked Columns for Antibody Purification

We now offer LigaTrap™ loose resins in prepacked columns as well. These FPLC chromatography columns are for use on the AKTA™ or other pressurized protein purification systems.

Size Available: 1 mL, 5 mL und 3×1 mL

Flow Rates: 1 mL Columns: 0.1 – 2.0 mL / min ; 5 mL Columns: 1.0 – 5.0 mL / min

LigaTrap prepacked column

General Overview

We offer LigaTrap™ products for antibody purification for various species and isotypes. You can choose from Ready-to-Use Kits, Resin or prepacked columns (1x1ml, 3x1ml und 1x5ml). A general overview is provided in the table below or try our Product Search.