Streptavidin-TRITC

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Overview
SKU 016-020-084 Protein Application Bio Assay, ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Immunoprecipitation, Western Blot
Conjugation Maximum Absorption Maximum Emission Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), in PBS (pH 7.6), lyophilisate
Dilution Flow Cytometry 2 – 5 µg/ml, Histo-/Cytochemistry 2 – 5 µg/ml
Intended Use Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Practical Implementation:
CAL2 labels the megakaryocytes in myeloproliferative neoplasms (essential thrombocythaemia (ET) and primary myelofibrosis (PMF)) with CALR mutation and enables to distinguish ET and PMF with CALR mutation from polycythemia vera (PV), from CALR mutation negative ET and PMF and from reactive bone marrow.
Why is CAL2 able to detect all known types of CALR mutations?
All types of CALR mutation result in a novel C-terminus. This harbors a common epitope expressed in all kinds of CALR mutations. The CAL2 antibody is directed against this common epitope. Therefore, it can be concluded that the CAL2 antibody is able to detect all CALR mutations.
CAL2 IHC of four PMF cases:
A and C: Selective staining of mutated CALR protein in megakaryocytes of two PMF cases, respectively in prefibrotic phase and in fibrotic phase, in which Sanger sequencing detected a CALR mutation.
B and D: Absent CAL2 staining in two PMF cases, respectively in prefibrotic and in fibrotic phase, both without molecularly detected mutated CALR. The fibrotic stroma remains unstained (C and D).
A
B
C
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References
- Stein, H et al. A new monoclonal antibody (CAL2) detects CALRETICULIN mutations in formalin-fixed and paraffin embedded bone marrow sections. Leukemia 2015, accepted for publication.
- Nangalia J et al. Somatic CALR Mutations in Myeloproliferative Neoplasms with Nonmutated JAK2. N Engl J Med 369(25): 2391-2405, 2013.
- Klampfl T et al. Somatic Mutations of Calreticulin in Myeloproliferative Neoplasms. N Engl J Med 369(25): 2379-2390, 2013.
More InformationDataSheet
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016-020-084 – Streptavidin-TRITC – 1 mgQuantity: 1 mg
Usual delivery time: 6 business days
169,00 € plus VAT
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