Goat IgG anti-Mouse IgG (Fc)-HRPO, MinX Hu,Bo,Ho
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SKU 115-035-071 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application Conjugation No Cross-reactivity (MinX) with Dilution Format Application Note
does not react with IgG, F(ab')2 Fragment, does not react with immunoglobulin light chains, IgG, Fc gamma-specific, useful for the detection of 25kD proteins in Western blotting after immunoprecipitation
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Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of mouse IgG heavy chain but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or non immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and horse serum proteins, but it may cross-react with immunoglobulins from other species.
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.