Goat Fab anti-Mouse IgG2b (Fc)-Alexa Fluor 488, MinX none
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Overview
SKU 115-547-187 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections)
Conjugation Maximum Absorption Maximum Emission No Cross-reactivity (MinX) with Dilution Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
Application Note recommended weight ratio (w/w) Fab:Prim.Ab 1:1 (3:1 molar ratio), suitable to complex with primary antibody in solution, titrate complex to optimal dilution for assay, vortex Prim.Ab+Fab and incubate 30 min at RT
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG2b but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or against non immunoglobulin serum proteins. The antibody may cross-react with other mouse IgG subclasses or with immunoglobulins from other species.
Conjugate
Alexa Fluor 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media.