Goat IgG anti-Alpaca IgG (H+L)-Biotin, MinX none
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Overview
SKU 128-065-003 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections)
Conjugation No Cross-reactivity (MinX) with Dilution ELISA 1:20,000 – 1:400,000, Flow Cytometry 1:200 – 1:1,000, Histo-/Cytochemistry 1:500 – 1:5,000, Immunocytochemistry (ICC): 1:200 – 1:1,000, Immunofluorescence 1:200 – 1:1,000, Western Blot (WB): 1:20,000 – 1:400,000
Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
Application Note Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule alpaca IgG, and with llama IgG. It also reacts with the light chains of other alpaca immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Conjugate
Biotin-SP is the trade name of Jackson ImmunoResearch for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.