Goat IgG anti-Alpaca IgG (VHH)-RPE, MinX Bo,Hu,Ms,Rb,Rt

533,00 

250 µl
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SKU: 128-115-230 Category: Tag:
  • Overview
    SKU 128-115-230
    Host Species

    IgG Form

    Species Reactivity

    Specificity

    Isotype

    Clonality (Mono-/Polyclonal)

    Application

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    Conjugation

    Maximum Absorption

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    Maximum Emission

    No Cross-reactivity (MinX) with

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    Dilution

    Format

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    Intended Use

    Product line / Topic

    Manufacturer / Brand

  • Datasheets and Downloads
  • Additional Product Information
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on antigen-binding assay, Western blot and/or ELISA, the antibody reacts with the VHH domain of heavy chain (HC) alpaca IgG, subclasses 2 and 3, and with the VHH domain of llama IgG, subclasses 2 and 3. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, human, mouse, rabbit, and rat serum proteins, but it may cross-react with immunoglobulins from other species.

    These antibodies react primarily with the Fc region, and are not recommended for detection of VHH antibodies.

    Conjugate

    Phycoerythrin (R-PE) is among several kinds of light-harvesting phycobiliproteins found in red, blue-green, and cryptomonad algae. We offer R-PE, the form found in red macrophytic algae (seaweed). After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. R-PE can be excited by light over a wide range of the visible spectrum is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates.

    It should be noted that the relatively high molecular weight of R-PE may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.

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