Goat IgG anti-Alpaca IgG2+IgG3-Alexa Fluor 594, MinX Bo,Hu,Ms,Rb,Rt
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Overview
SKU 128-585-229 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections)
Conjugation Maximum Absorption Maximum Emission No Cross-reactivity (MinX) with Dilution Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM PBS (pH 7.6), lyophilisate
Application Note Heavy chain (HC) alpaca IgG, subclasses 2 and 3, It also reacts with HC llama IgG, subclasses 2 and 3.
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with heavy chain (HC) alpaca IgG, subclasses 2 and 3. It also reacts with HC llama IgG, subclasses 2 and 3. No antibody was detected against alpaca or llama IgG1, light chains or non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, human, mouse, rabbit, and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
Alexa Fluor 594-conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. They are brighter, more photostable, and more hydrophilic than Texas Red conjugates. Alexa Fluor 594 conjugates are brighter than red-fluorescing conjugates, and they provide more color separation from green-fluorescing dyes than DyLight 549, Cy3, and TRITC conjugates. They are the best choice for immunofluorescence detection in the deep-red region of the visible spectrum.