Anti-N6-Methyladenosine (m6A) from Rabbit – unconj.
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Overview
SKU 31-1248-00 Specificity Host Species Isotype Clone Clonality (Mono-/Polyclonal) Conjugation Format 50% Glycerol / PBS with 1% BSA and 0.09% sodium azide, from culture supernatant, Protein A affinity purified antibody
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Images
Dot blot of Lambda DNA without or with m6A, usinganti-m6A antibody (RM362). The membrane waspre-spotted with 5, and 0.5 ng/dot of lambda DNA.m6A-free DNA was isolated from bacteriophagelambda grown in an E. coli host-deficient in adeninemethylase (dam-). MeRIP was performed using M6A and control RNAfrom NEB (E1610). Antibodies (Anti-m6A RM362and Anti-PEG RM105) were mixed for a short time,with Protein A beads before the addition of m6Alabelled and non- labelled RNA (in equal amounts).Captured RNA was then
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