Bovine IgG anti-Goat IgG (H+L)-Alk. Phos., MinX Bo,Ck,Gp,Hs,Ho,Hu,Ms,Rb,Rt
-
Overview
SKU 805-055-180 Host Species IgG Form Species Reactivity Specificity Isotype Clonality (Mono-/Polyclonal) Application ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen sections), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Western Blot
Conjugation No Cross-reactivity (MinX) with Bovine, Chicken, Guinea Pig, Hamster syrian, Horse, Human, Mouse, Rabbit, Rat
Dilution ELISA 1:5,000 – 1:50,000, Histo-/Cytochemistry 1:500 – 1:5,000, Western Blot (WB): 1:5,000 – 1:50,000
Format 0.05% NaN3, 15 mg/ml BSA (IgG- and Protease-Free), 250 mM NaCl, affinity purified by antigen-specific affinity chromatography, in 10 mM Tris-HCI (pH 8.0), lyophilisate
Intended Use Product line / Topic Manufacturer / Brand - Datasheets and Downloads
-
Additional Product Information
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule goat IgG. It also reacts with the light chains of other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with goat, chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.