Pathogen DNA Isolation Kit (Bacteria & Fungi), RUO (50 tests)
SKU 9037050 Article Group Product Name Application Test Principle Contents Sample Material Sample Volume Time/Protocol Downstream Applications Intended Use Size Manufacturer / Brand
- Additional Product InformationThe RTA Pathogen DNA Kit allows the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of fungi and gram positive and gram negative bacterial species. With the customized lysis buffer, RTA Pathogen DNA isolation kit provides DNA elution of pathogenic organisms from difficult materials such as food, soil, environmental specimens. This kit delivers an inhibitor-free final product.
The RTA Pathogen DNA kit uses optimized lysis condition and up to 1 x 109 bacterial cells can be processed for each column. Pathogen cells are grown to log-phase and harvested or harvested directly from the sample. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted, and the sample is applied to a RTA DNA spin column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or Elution Buffer. All process steps are completed in 30 minutes. Purified DNA can be directly used in downstream applications without the need for further purification.