Anti-CD45R (Ms) from Rat (Clone: GHH45) for mouse FFPE tissue
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Gene Ptprc encodes for the Protein Tyrosine Phosphatase (PTP) family member CD45, also known as LCA (Leucocyte Common Antigen). This type 1 glycoprotein contains a single variable extracellular domain, a transmembrane segment and two intracellular domains, which are both required for full enzymatic activity, followed by a short tail at the C-terminus. The PTPase plays an important role in regulation of antigen receptor signaling and development of T- and B-cells. Several splice variants of CD45 mRNA exist, affecting the composition of the heavily glycosylated extracellular segment at the N-terminus. Clone GHH45 is raised against a conserved region at the C-terminus of CD45 and detects three murine isoforms of leukocyte receptor CD45R (alternative names: B220, Ly-5 and Protein tyrosine phosphatase receptor type C). In addition, this region is particularly suitable for antibody-antigen recognition in standard FFPE-tissue sections.
CD45R is predominantly expressed on the surface of B-cells (level of expression increases from early pro-B-cell stage to ma-ture B-cells, but diminishes with final differentiation into plasma cells) and activated B-cells, as well as on a subset of T-cells, dendritic cells and NK-cells. Infectious, autoimmune and malignant diseases are associated with altered CD45 expression in humans.
Immunohistochemistry of mouse CD45R in formalin-fixed paraffin-embedded tissue sections
(A) Mouse Spleen (40x)
(B) Mouse Kidney (40x)
(C) Mouse Lung (40x)
(D) Mouse Liver (40x)
Staining of murine FFPE tissues using 10 µg/ml anti-CD45R antibody clone GHH45 and ABC-system. Immunoreactive cells have been revealed with 3,3’-Diaminobenzidine (DAB) as chromagen. Nuclei have been counterstained with haematoxylin (blue). (Picture courtesy of Christel Bonnas, Synaptic Systems, Göttingen, Germany).
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