Anti-CD45R (Ms) from Rat (Clone: GHH45) for mouse FFPE tissue


200 µl

Estimated delivery time: 6 business days

SKU: DIA-450 Categories: , Tags: ,
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    SKU DIA-450

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    Gene Ptprc encodes for the Protein Tyrosine Phosphatase (PTP) family member CD45, also known as LCA (Leucocyte Common Antigen). This type 1 glycoprotein contains a single variable extracellular domain, a transmembrane segment and two intracellular domains, which are both required for full enzymatic activity, followed by a short tail at the C-terminus. The PTPase plays an important role in regulation of antigen receptor signaling and development of T- and B-cells. Several splice variants of CD45 mRNA exist, affecting the composition of the heavily glycosylated extracellular segment at the N-terminus. Clone GHH45 is raised against a conserved region at the C-terminus of CD45 and detects three murine isoforms of leukocyte receptor CD45R (alternative names: B220, Ly-5 and Protein tyrosine phosphatase receptor type C). In addition, this region is particularly suitable for antibody-antigen recognition in standard FFPE-tissue sections.
    CD45R is predominantly expressed on the surface of B-cells (level of expression increases from early pro-B-cell stage to ma-ture B-cells, but diminishes with final differentiation into plasma cells) and activated B-cells, as well as on a subset of T-cells, dendritic cells and NK-cells. Infectious, autoimmune and malignant diseases are associated with altered CD45 expression in humans.

    Immunohistochemistry of mouse CD45R in formalin-fixed paraffin-embedded tissue sections
    Immunohistochemical staining (IHC) with anti-CD45R Antibody (clone GHH45) - dianova

    (A) Mouse Spleen (40x)

    Immunohistochemical staining (IHC) with anti-CD45R Antibody (clone GHH45) - dianova

    (B) Mouse Kidney (40x)

    (C) Mouse Lung (40x)

    (D) Mouse Liver (40x)

    Staining of murine FFPE tissues using 10 µg/ml anti-CD45R antibody clone GHH45 and ABC-system. Immunoreactive cells have been revealed with 3,3’-Diaminobenzidine (DAB) as chromagen. Nuclei have been counterstained with haematoxylin (blue). (Picture courtesy of Christel Bonnas, Synaptic Systems, Göttingen, Germany).

    General References:

    1. Coffman,R.L. (1982). Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell develop-ment.Immunol.Rev.69:5
    2. Osmond, D. G., Rolink, A., and Melchers, F. (1998). Murine B lymphopoiesis: Towards a unified model. Immunol Today 19, 65–8.
    3. Hardy, R. R., Kincade, P. W., and Dorshkind, K. (2007). The protean nature of cells in the B lymphocyte lineage. Immunity 26, 703–14.
    4. Blasius, A. L., Barchet, W., Cella, M., and Colonna, M. (2007). Development and function of murine B220+CD11c+NK1.1+ cells identify them as a subset of NK cells. J Exp Med 204, 2561–8.
    5. Vosshenrich, C. A., Lesjean-Pottier, S., Hasan, M., Richard-Le Goff, O., Corcuff,E., Mandelboim, O., and Di Santo, J. P. (2007). CD11cloB220+ interferon-producing killer dendritic cells are activated natural killer cells. J Exp Med 204, 2569–78.
    6. Hermiston ML, Zikherman J, Zhu JW. CD45, CD148, and Lyp/Pep: critical phosphatases regulating Src family kinase signaling networks in immune cells. Immunological Reviews; 2009; 228:288–311.Tchilian EZ, Beverley PC. Altered CD45 expression and disease. Trends in immunology 2006 Mar;27(3):146–53.

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