ExKine Cytoplasmic Protein Extraction Kit (Mammals), RUO (200 tests)
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Overview
SKU KTP3003-2 Article Group Product Name Species Reactivity Description from mammalian cultured cells or tissues, stepwise separation and preparation of cytoplasmic extracts
Contents Cytoplasmic Solution A (CES A), Cytoplasmic Solution B (CES B), DTT (500X), Protease Inhibitor (100X)
Intended Use Temperature - Storage Temperature - Transport Size Manufacturer / Brand - Datasheets and Downloads
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Additional Product Information
The preparation of an extract from Cytoplasm is often the first step in studying Cytoplasmic proteins and their interactions. The resulting preparation can be used directly in Western blotting, Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.
Abbkine ExKine™ Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of cytoplasmic extracts from mammalian cultured cells or tissues. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the nuclear fraction is removed. Non-denatured, active proteins are purified in less than two hours.
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