31-1004-00 – Kaninchen IgG anti-Maus IgG2b (Fc) (RM108)-unkonj., MinX Hu,Rt,Go – 100 µg

Art.-Nr.
31-1004-00

Wirtsspezies
Kaninchen
IgG-Form
IgG Gesamtmolekül
Spezies-Reaktivität
Maus
Spezifität
IgG2b (IgG Fc gamma 2b)
Isotyp
IgG Gesamtmolekül
Klon
RM108
Klonalität (Mono-/Polyklonal)
monoklonal
Anwendung
ELISA (Enzyme Linked Immunosorbent Assay)
Durchflusszytometrie (Flow Cytometry)
Western Blot
Immunpräzipitation
Immuncytochemie
Immunhistochemie (IHC)
WB (nichtreduziert)
Konjugation
unkonjugiert
Keine Kreuzreaktivität (MinX) mit
Ratte
Ziege
Human
Format
ProteinA/G-gereinigt (aus Kulturüberstand)
Anwendungshinweis
Antikörperkonzentration: 1mg/ml
Reacts to the Fc region of mouse IgG2b
No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG
Zweckbestimmung
nur für Forschungszwecke
Produktlinie / Thema
RevMab / Sekundärantikörper
Temperatur – Lagerung
-20°C
Hersteller / Marke
RevMAb Biosciences

Western blot detection (WB) with anti-IgG2b (IgG Fc gamma 2b) Antibody (clone RM108) - RevMAb Biosciences

Western blot of nonreduced (-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/mL of RevMAb clone RM108. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced γ2b form (~50 kDa).

ELISA with anti-IgG2b (IgG Fc gamma 2b) Antibody (clone RM108) - RevMAb Biosciences

ELISA of mouse immunoglobulins shows RM108 reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA with anti-IgG2b (IgG Fc gamma 2b) Antibody (clone RM108) - RevMAb Biosciences

A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Weitere Informationen

Menge: 100 µg

Regellieferzeit: 12 Tage


298,00  zzgl. MwSt.
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