31-1024-02 – Kaninchen IgG anti-Human IgE (RM122)-Biotin, – 50 µg

Art.-Nr.
31-1024-02

Wirtsspezies
Kaninchen
IgG-Form
IgG Gesamtmolekül
Spezies-Reaktivität
Human
Spezifität
IgE (Epsilon-Kette/ Fc-Fragment)
Isotyp
IgG Gesamtmolekül
Klon
RM122
Klonalität (Mono-/Polyklonal)
monoklonal
Anwendung
ELISA (Enzyme Linked Immunosorbent Assay)
Durchflusszytometrie (Flow Cytometry)
Immuncytochemie
Immunhistochemie (IHC)
Konjugation
Biotin
Format
ProteinA/G-gereinigt (aus Kulturüberstand)
Anwendungshinweis
Antikörperkonzentration: 1mg/ml
Reacts to human IgE
No cross reactivity with human IgG, IgM, IgD, or IgA
Zweckbestimmung
nur für Forschungszwecke
Produktlinie / Thema
RevMab / Sekundärantikörper
Temperatur – Lagerung
-20°C
Hersteller / Marke
RevMAb Biosciences

ELISA with anti-IgE (Epsilon-Kette/ Fc-Fragment) Antibody (clone RM122) - RevMAb Biosciences

Sandwich ELISA using RM122 as the capture antibody (25ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

ELISA with anti-IgE (Epsilon-Kette/ Fc-Fragment) Antibody (clone RM122) - RevMAb Biosciences

ELISA of human immunoglobulins shows RM122 reacts only to human IgEλ from human myeloma plasma and the IgE from human plasma. No cross reactivity with Human IgG, IgM, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL or 10 ng/mL of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA with anti-IgE (Epsilon-Kette/ Fc-Fragment) Antibody (clone RM122) - RevMAb Biosciences

A titer ELISA using RM122. The plate was coated with different amounts of human IgE. A serial dilution of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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31-1024-02 - Kaninchen IgG anti-Human IgE (RM122)-Biotin, - 50 µg

Menge: 50 µg

Regellieferzeit: 12 Tage


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