DIA-310 – Anti-CD31 (Ms) aus Ratte (Klon: SZ31) für Paraffingewebe aus Maus – 500 µl

Art.-Nr.
DIA-310

Spezifität
CD31 (PECAM-1)
Spezies-Reaktivität
Maus
Schwein
Wirtsspezies
Ratte
Isotyp
IgG2a
Klon
SZ31
Klonalität (Mono-/Polyklonal)
monoklonal
Anwendung
Gefrierschnitte (IHC-F)
Immunhistochemie (Paraffingewebe)
Western Blot
Immunhistochemie (IHC)
Konjugation
unkonjugiert
Format
Lyophilisat
gereinigter Antikörper (aus Kulturüberstand)
Produktlinie / Thema
CD-Marker
Angiogenese
Immunhistochemie
Zweckbestimmung
nur für Forschungszwecke
Temperatur – Lagerung
2-8°C
Suchcode
DIA310
Hersteller / Marke
dianova

Reactivity:

Der Antikörper-Klon SZ31 reagiert als erster Antikörper spezifisch mit CD31 in standardmässig Formalin-fixierten Paraffin-eingebetteten (FFPE) Mausgeweben.

Background:

CD31, auch bekannt als PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1), wird auf der Oberfläche embryonaler und adulter Endothelzellen konstitutiv exprimiert. Sowie auf Zelloberflächen von Monozyten, Neutrophilen, Thrombozyten und verschiedener Untergruppen der T-Zellen. Zudem konnte das Molekül auf hämatopoetischen Stammzellen aus dem Knochenmark und embryonalen Stammzellen nachgewiesen werden. Cluster of Differentiation 31 (CD31) ist ein integrales Membranglykoprotein (130 kDa), welches als Mitglied der Immunglobulin-Superfamilie an der Vermittlung der Zell-Zell-Adhäsion beteiligt ist. Die von CD31 vermittelte endotheliale Zell-Zell-Interaktion, spielt hierbei eine entscheidende Rolle in der Angiogenese. Das Adhäsionsmolekül gilt als einer der wichtigsten Marker in der humanen Angiogenese, Studien zufolge kann der Nachweis von CD31 auf ein Tumorrezidiv deuten. Pathophysiologische Untersuchungen von CD31 in Maus-Modellsystemen waren bisher nur begrenzt möglich, weil Standard-FFPE Gewebe ausgeschlossen werden mussten. Der Ratten-Klon SZ31 (Anti-CD31) löst diese Probleme. Unser monoklonaler Antikörper erlaubt erstmals immunhistochemische Analysen von CD31 in Formalin-fixierten Paraffingeweben (FFPE) der Maus und überzeugt durch beeindruckende Qualität der mikroskopischen Aufnahmen.

Immunhistochemischer Nachweis von Maus-CD31 (PECAM-1) in Formalin-fixierten, Paraffin-eingebetteten Gewebeschnitten

Der monoklonale Antikörper Klon SZ31 reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren von:

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse aorta (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren der Mausaorta (FFPE Gewebe).

aorta

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse aorta origin (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren der Mausaorta (FFPE Gewebe).

aortic origin

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse endocardium (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren des Endokard aus der Maus (FFPE Gewebe).

endocardium

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse small intestine (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren des Mäuse Dünndarm (FFPE Gewebe).

small intestine

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

small intestine

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

colon

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

brain

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

lymph nodes

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

bone marrow

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

mesenterial vessels

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

kidney

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

pancreas

(Bilder mit freundlicher Genehmigung von Prof. Dr. Robert Klopfleisch, Institute of Pathology, Department of Veterinary Pathology, Berlin)

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

Lung

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

Muscle

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

Spinal

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

Liver

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

Adenocarcinoma

Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

Adenocarcinoma

(Bilder mit freundlicher Genehmigung von Prof. Dr. H. Stein, Institute of Pathology, Charité Campus Benjamin Franklin, Berlin)

Western Blot-Analyse

Immunoblot mit Zellextrakten aus Mauslunge, J558L Zellen und m-Lend Zellen. Antikörper-Klon SZ31 (DIA-310 1:500) wurde zusammen mit einem Ziege Anti-Ratte-HRP Sekundärantikörper (1:10.000) zum Nachweis von CD31 verwendet.

Western blot detection (WB) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

Spur:

  1. J558 Zellen (CD31-), 25μg Lysat
  2. Mauslunge, 25μg Lysat
  3. blank
  4. m-Lend Zellen (CD31+), 12.5μg Lysat

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