DIA-310 – Anti-CD31 (Ms) aus Ratte (Klon: SZ31) für Paraffingewebe aus Maus – 500 µl

Art.-Nr.
DIA-310

Spezifität
CD31 (PECAM-1)
Spezies-Reaktivität
Maus
Schwein
Wirtsspezies
Ratte
Isotyp
IgG2a
Klon
SZ31
Klonalität (Mono-/Polyklonal)
monoklonal
Anwendung
Gefrierschnitte (IHC-F)
Immunhistochemie (Paraffingewebe)
Western Blot
Immunhistochemie (IHC)
Konjugation
unkonjugiert
Format
Lyophilisat
gereinigter Antikörper (aus Kulturüberstand)
Produktlinie / Thema
CD-Marker
Angiogenese
Immunhistochemie
Zweckbestimmung
nur für Forschungszwecke
Suchcode
DIA310
Hersteller / Marke
dianova

Reactivity

Antibody clone SZ31 is the first antibody which reacts specifically with murine CD31 in formalin-fixed paraffin-embedded tissue sections.
CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) is expressed constitutively on the surface of embryonic and adult endothelial cells. It is also expressed on cell surfaces of monocytes, neutrophils, platelets and certain T-cell subsets. It has been detected on bone marrow-derived hematopoetic stem cells and embryonic stem cells. CD31 is a 130kDa integral membrane glycoprotein and as a member of the immunoglobulin superfamily involved in the mediation of cell-to-cell adhesion. CD31-mediated endothelial cell-cell interactions play a major role in angiogenesis. Studies have shown CD31 to be a superior marker in human angiogenesis, which reportedly predicts tumor recurrence. Pathophysiological studies of CD31 in murine model systems had limitations because standard formalin-fixed sections were excluded. The clone SZ31 eliminates these restrictions by allowing high quality immunohistochemical analysis of standard formalin-fixed paraffin sections in mice.

Immunohistochemistry of mouse CD31 (PECAM-1) in formalin-fixed paraffin-embedded tissue sections

The monoclonal antibody clone SZ31 reacts specifically with endothelial cells in vessels and capillaries of:

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse aorta (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren der Mausaorta (FFPE Gewebe).

aorta

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse aorta origin (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren der Mausaorta (FFPE Gewebe).

aortic origin

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse endocardium (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren des Endokard aus der Maus (FFPE Gewebe).

endocardium

The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse small intestine (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren des Mäuse Dünndarm (FFPE Gewebe).

small intestine


small intestine


colon


brain


lymph nodes


bone marrow


mesenterial vessels


kidney


pancreas

(pictures courtesy of Prof. Dr. Robert Klopfleisch, Institute of Pathology, Department of Veterinary Pathology, Berlin, Germany)


Lung


Muscle


Spinal


Liver


Adenocarcinoma


Adenocarcinoma

(pictures courtesy of Prof.Dr.H.Stein, Institute of Pathology, Charité Campus Benjamin Franklin, Berlin, Germany)

Western blot analysis

Immunoblot of extracts from murine lung, J558L cells and m-Lend cells using CD31 rat monoclonal antibody clone SZ31 (DIA-310 1:5.000) and goat anti-rat-HRP antibody (1:10.000).

Lane:

  1. J558 cells (CD31-), 25μg lysate
  2. murine lung, 25μg lysate
  3. blank
  4. m-Lend cells (CD31+), 12.5μg lysate

References

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    Cardiomyocyte-specific prolyl-4-hydroxylase domain 2 knock out protects from acute myocardial ischemic injury.
    J.Biol.Chem. 286: 11185-11194, 2011
  2. Kröger C, Vijayaraj P, Reuter U, Windoffer R, Simmons D, Heukamp L, Leube R, Magin TM. Placental vasculogenesis is
    regulated by keratin-mediated hyperoxia in murine decidual tissues. Am.J.Pathol. 178(4): 1587-1590, 2011
  3. Jiang J, Thyagarajan-Sahu A, Krchnák V, Jedinak A, Sandusky GE, et al. NAHA, a Novel Hydroxamic Acid-Derivative,
    Inhibits Growth and Angiogenesis of Breast Cancer In Vitro and In Vivo. PLoS ONE 7(3): e34283, 2012
  4. Copeland BT, Bowman MJ, Ashman LK. Genetic Ablation of the Tetraspanin CD151 Reduces Spontaneous Metastatic
    Spread of Prostate Cancer in the TRAMP Model. Mol Cancer Res. 11(1):95-105, 2013
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    of l e n a l i d o m i d e in a novel xenograft mouse model of human blastic NK cell lymphoma/blastic plasmacytoid dendritic
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    Airoldi I. Absence of IL-12Rβ2 in CD33(+)CD38(+) pediatric acute myeloid leukemia cells favours progression in
    NOD/SCID/IL2RϒC-deficient mice. Leukemia 26(2):225-35,2012