DIA-PX8-OD – Anti-Paired box protein pax-8 (PAX-8) (Hu) aus Maus (JAX8) – unkonj. – 100 µl

Art.-Nr.
DIA-PX8-OD

Spezifität
Paired box protein pax-8 (PAX-8)
Spezies-Reaktivität
Human
Wirtsspezies
Maus
Isotyp
IgG2a
Klon
JAX8
Klonalität (Mono-/Polyklonal)
monoklonal
Anwendung
Immunhistochemie (Paraffingewebe)
ELISA (Enzyme Linked Immunosorbent Assay)
Western Blot
Immunhistochemie (IHC)
Konjugation
unkonjugiert
Verdünnung
Immunhistochemie (IHC): 1:100 – 1:400
Format
ProteinA/G-gereinigter Antikörper
Konzentrat
1% BSA
in Tris Puffer (pH 7,3-7,7)
< 0,1% NaN3
Immunogen
Recombinant Human PAX8
Zweckbestimmung
nur für Forschungszwecke
Temperatur – Lagerung
2-8°C
Temperatur – Transport
gekühlt
Hersteller / Marke
ONCOdianova
Alias
PAX-8
Paired box protein pax-8

Ultra-validated anti-PAX8 IHC-Gallery

Clone JAX8 is ultra-validated for IHC detection of PAX8 transcription factor in routine-fixed FFPE tissue sections.

Oncodianova future Biomarkers

ONCOdianova develops new antibodies for IHC detection of cancer biomarkers in FFPE-tissues.

Reactivity

Mouse monoclonal anti-PAX8 antibody clone JAX8 is suitable for the immunohistological detection of PAX8 in routine-fixed paraffin embedded tissue sections.
PAX8 is a member of the paired box (PAX) family of transcription factors involved the regulation of early development of the thyroid gland, kidney, and Müllerian tract. PAX8 plays a central role for the expression of thyroid-specific genes and thus, in development of thyroid follicular cells. Mutations in the PAX-8 gene are linked to thyroid follicular carcinomas. In the develop-ing kidney PAX8 is important for renal vesicle formation.
PAX8 is highly expressed in several neoplasms: Epithelial tumors of the thyroid and parathyroid glands, kidney, thymus, pan-creatic neuroendocrine tumors and female genital tract. Follicular and papillary thyroid carcinoma are almost always PAX8 positive (while medullary thyroid carcinoma is negative, but anaplastic carcinoma is positive in most cases). PAX8 is also found in almost all cases of endometrial carcinoma and ovarian serous, endometrioid, transitional and clear cell carcinoma. Moreover, PAX8 is found in most cases of renal cell carcinoma (all types) and in oncocytoma as well as in thymic tumours. Difefferent reports have shown that adenocarcinomas of lung and breast are negative for PAX8-expression. Also, PAX-8 is not found in the epithelial cells of the breast, lung, mesothelium, stomach, colon, pancreas.
PAX 8 is a useful IHC marker with a wide range of diagnostic applications and appears to be the most specific and sensitive marker for renal cell carcinoma and ovarian non-mucinous carcinoma.

Immunohistochemistry of human PAX8 in routine formalin-fixed paraffin-embedded (FFPE) tissue samples

A: Strong nuclear PAX8 positivity in a serous ovarian carcinoma.
B: Endometrioid endometrial carcinoma with strong PAX8 staining (nuclear and cytoplasmic).
C: Clear cell renal cell carcinoma with nuclear PAX8 staining.
D: Strong nuclear PAX8 positivity in a bengin thyroid adenoma.


(A) Serous ovarian carcinoma


B) Endometrioid endometrial carcinoma


(C) Clear cell renal cell carcinoma


(D) Bengin thyroid adenoma

(Pictures kindly provided by Christoph Fraune & Christina Merenkow, Department of Pathology, University Hospital Eppendorf (UKE), Hamburg, Germany)

References

  1. Marques AR et al. (2002) Expression of PAX8 PPAR gamma 1 rearrangements on both follicular thyroid carcinomas and andenomas. J Clin Endocrinol Metab. 87: 3947-3952
  2. Nikiforova MN et al. (2002) PAX8-PPARgamma rearrangement in thyroid tumors: RT-PCR and immunohistochemi-cal analyses. Am J Surg Pathol. 26: 1016-1023
  3. Zhang P, et al. (2006) Immunohistochemical analysis of thyroid-specific transcription factors in thyroid tumors. Pathol Int. 56: 240-245
  4. Bowen NJ, et al. (2007) Emerging roles for PAX8 in ovarian cancer and endosalpingeal development. Gynecol On-col. 104: 331-337
  5. Nonaka, D et al. (2008) Diagnostic utility of thyroid transcription factors Pax8 and TTF-2 (Fox E1) in thyroid epithelial neoplasms. Mod Pathol. 21: 192-200
  6. Nonaka D et al. (2008) Expression of pax8 as a useful marker in distinguishing ovarian carcinomas from mammary carcinomas. Am J Surg Pathol. 32: 1566-157
  7. Tong GX et al. (2009) Expression of PAX8 in normal and neoplastic renal tissues: an immunohistochemical study. Mod Pathol. 22: 1218-1227
  8. Laury AR et al. (2011) A comprehensive analysis of PAX8 expression in human epithelial tumors. Am J Surg Pathol. 35: 816-826
  9. Ozcan A et al. (2011) PAX 8 expression in non-neoplastic tissues, primary tumors, and metastatic tumors: a compre-hensive immunohistochemical study. Mod Pathol.; 24: 751-764

Menge: 100 µl

Regellieferzeit: 10 Tage


328,00  zzgl. MwSt.
Versandkostenfrei innerhalb Deutschlands