Ziege IgG anti-Human IgG (Fc)-Alk. Phos., MinX Bo,Ho,Ms
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Übersicht
Artikelnummer 109-055-098 Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klonalität (Mono-/Polyklonal) Anwendung ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe), Western Blot
Konjugation Keine Kreuzreaktivität (MinX) mit Verdünnung ELISA 1:5.000 – 1:50.000, Histo-/Zytochemie 1:500 – 1:5.000, Western Blot (WB): 1:5.000 – 1:50.000
Format 0,05% NaN3, 15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM Tris-HCI (pH 8,0), Lyophilisat
Anwendungshinweis Zweckbestimmung Produktlinie / Thema Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse, and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.