Maus IgG anti-Ziege IgG (L) (20B07-02A09)-Biotin, MinX Ho,Hu,Ms,Rb,Rt
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Übersicht
Artikelnummer 205-062-176 Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klon Klonalität (Mono-/Polyklonal) Anwendung Durchflusszytometrie (Flow Cytometry), ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe), Western Blot
Konjugation Keine Kreuzreaktivität (MinX) mit Verdünnung Durchflusszytometrie 1:200 – 1:1.000, ELISA 1:20.000 – 1:400.000, Immunfluoreszenz 1:200 – 1:1.000, Immunhistochemie (IHC): 1:500 – 1:5.000, Western Blot (WB): 1:20.000 – 1:400.000
Format 0,05% NaN3, 15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM PBS (pH 7,6), Lyophilisat
Zweckbestimmung Produktlinie / Thema Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on goat IgG and with those common to other goat immunoglobulins. The antibody does not react with the heavy chain of goat IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with horse, human, mouse, rabbit, and rat immunoglobulins, but it may cross-react with immunoglobulins from other species.
Conjugate
Biotin-SP is the trade name of Jackson ImmunoResearch for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.