Maus IgG anti-Schaf IgG (L) (9A12-2E10)-HRPO, MinX Ho,Hu,Ms,Rb,Rt
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Übersicht
Artikelnummer 213-032-177 Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klon Klonalität (Mono-/Polyklonal) Anwendung ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe), Western Blot
Konjugation Keine Kreuzreaktivität (MinX) mit Verdünnung ELISA 1:5.000 – 1:100.000, Histo-/Zytochemie 1:500 – 1:5.000, Western Blot (ECL): 1:10.000 – 1:200.000, Western Blot (non-ECL): 1:5.000 – 1:100.000
Format 15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM PBS (pH 7,6), Lyophilisat
Zweckbestimmung Produktlinie / Thema Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on sheep IgG and with those common to other sheep immunoglobulins. The antibody does not react with the heavy chain of sheep IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, horse, human, mouse, rabbit, and rat immunoglobulins, but it may cross-react with immunoglobulins from other species.
Conjugate
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.