Ziege IgG anti-Meerschweinchen IgG (F(ab’)2)-Alk. Phos., MinX keine

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab’)2/Fab portion of guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against the Fc portion of guinea pig IgG or against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Conjugate

Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.