Ziege IgG anti-Alpaka IgG2+IgG3-Alk. Phos., MinX Bo,Hu,Ms,Rb,Rt
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Übersicht
Artikelnummer 128-055-229 Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klonalität (Mono-/Polyklonal) Anwendung Konjugation Keine Kreuzreaktivität (MinX) mit Verdünnung ELISA 1:5.000 – 1:50.000, Western Blot (WB): 1:5.000 – 1:50.000
Format 0,05% NaN3, 10 mM Tris-HCl (pH 8,0), 15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, Lyophilisat
Anwendungshinweis Reagiert auch mit Schwere-Ketten (HC) IgG-Antikörper des Llamas, Subklassen 2 und 3., Schwere-Ketten (HC) IgG-Antikörper des Alpkakas, Subklassen 2 und 3
Zweckbestimmung Produktlinie / Thema Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with heavy chain (HC) alpaca IgG, subclasses 2 and 3. It also reacts with HC llama IgG, subclasses 2 and 3. No antibody was detected against alpaca or llama IgG1, light chains or non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, human, mouse, rabbit, and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.