Ziege IgG anti-Alpaka IgG2+IgG3-Biotin, MinX Bo,Hu,Ms,Rb,Rt
exkl. MwSt. Versandkostenfrei innerhalb Deutschlands
Voraussichtliche Lieferzeit: 6 Werktage
Artikelnummer 128-065-229 Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klonalität (Mono-/Polyklonal) Anwendung
Durchflusszytometrie (Flow Cytometry), ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe)
Konjugation Keine Kreuzreaktivität (MinX) mit Verdünnung
Durchflusszytometrie 1:200 – 1:1.000, ELISA 1:20.000 – 1:400.000, Histo-/Zytochemie 1:500 – 1:5.000, Immuncytochemie (ICC): 1:200 – 1:1.000, Immunfluoreszenz 1:200 – 1:1.000, Western Blot (WB): 1:20.000 – 1:400.000
Format Anwendungshinweis Zweckbestimmung Produktlinie / Thema Hersteller / Marke
- Weitere ProduktinformationenWhole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with heavy chain (HC) alpaca IgG, subclasses 2 and 3. It also reacts with HC llama IgG, subclasses 2 and 3. No antibody was detected against alpaca or llama IgG1, light chains or non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, human, mouse, rabbit, and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Biotin-SP is the trade name of Jackson ImmunoResearch for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.