Ziege IgG anti-Alpaka IgG (H+L)-RPE, MinX Hu,Ms,Rb,Rt
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Übersicht
Artikelnummer 128-115-160 Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klonalität (Mono-/Polyklonal) Anwendung Durchflusszytometrie (Flow Cytometry), Immuncytochemie, Immunfluoreszenz
Konjugation Maximale Anregung Maximale Emission Keine Kreuzreaktivität (MinX) mit Verdünnung Format 0,05% NaN3, 15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM PBS (pH 7,6), Lyophilisat
Anwendungshinweis Zweckbestimmung Produktlinie / Thema Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule alpaca IgG, and with llama IgG. It also reacts with light chains of other alpaca immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, human, mouse, rabbit and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
Phycoerythrin (R-PE) is among several kinds of light-harvesting phycobiliproteins found in red, blue-green, and cryptomonad algae. We offer R-PE, the form found in red macrophytic algae (seaweed). After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. R-PE can be excited by light over a wide range of the visible spectrum is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates.
It should be noted that the relatively high molecular weight of R-PE may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.