Anti-Monomethyl-Histone H3 (Lys4) (alle) aus Kaninchen (RM140) – unkonj.
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Übersicht
Artikelnummer 31-1046-00 Spezifität Spezies-Reaktivität Wirtsspezies Isotyp Klon Klonalität (Mono-/Polyklonal) Anwendung ELISA (Enzyme Linked Immunosorbent Assay), Multiplex, Western Blot
Konjugation Format Produktlinie / Thema Zweckbestimmung Temperatur - Lagerung Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
RM140 specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM140 at 0.5 ug/mL, showed a band of histone H3 monomethylated at Lysine 4 (K4me1) in HeLa cells.
ChIP performed on HeLa cells using H3K4me1 antibody (RM140, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Monomethyl-Histone H3 (Lys4) Rabbit mAb RM140 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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Bilder
Fig.-1-in-CofA-RM140 fig2-WB2 fig3-RM140 Fig4_RM140_ICC