Anti-Histone H3 (Unmodified Lys4) (alle) aus Kaninchen (RM186) – unkonj.
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Übersicht
Artikelnummer 31-1086-00 Spezifität Spezies-Reaktivität Wirtsspezies Isotyp Klon Klonalität (Mono-/Polyklonal) Anwendung Chromatin-Immunpräzipitation, ELISA (Enzyme Linked Immunosorbent Assay), Multiplex, Western Blot
Konjugation Format Anwendungshinweis Does not recognize acetylated or methylated Lys4, The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3
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RM186 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.
Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of histone H3 with unmodified Lys4.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody (clone RM186) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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