Anti-Phospho-VEGFR-2 (Tyr1059) (Hu,Ms,Rt) aus Kaninchen (pAk) – unkonj.
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Übersicht
Artikelnummer AF3279-02 Spezifität Spezies-Reaktivität Wirtsspezies Isotyp Klon Klonalität (Mono-/Polyklonal) Anwendung ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Western Blot
Konjugation Verdünnung Immuncytochemie (ICC): 1:100 – 1:500, Immunfluoreszenz 1:100 – 1:500, Western Blot (WB): 1:500 – 1:2.000
Format 0,02% NaN3, 150 mM NaCl, 1mg/ml Antikörper, 50% Glycerol, affinitätsgereinigter Antikörper (aus Antiserum), gereinigt durch sequentielle Chromatographie an Phospho- und Nicht-Phospho-Peptid-Affinitätssäulen, in PBS without Mg2+ and Ca2+ (pH 7,4)
Zweckbestimmung Temperatur - Lagerung Temperatur - Transport Hersteller / Marke Uniprot_ID Alias A Type III Receptor Tyrosine Kinase, CD309, Fetal Liver Kinase-1, FLK1, KDR, Kinase Insert Domain Receptor, Phospho-VEGFR-2 (Tyr1059), Vascular Endothelial Growth Factor Receptor 2, VEGFR, VEGFR-2, VEGFR2
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Weitere Produktinformationen
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Phospho-VEGFR2 (Tyr1059) Antibody detects endogenous levels of VEGFR2 only when phosphorylated at Tyrosine 1059