Anti-CD31 (Ms) aus Ratte (Klon: SZ31) für Paraffingewebe aus Maus

402,00 

500 µl

Voraussichtliche Lieferzeit: 1 Werktag

Artikelnummer: DIA-310 Kategorien: , Schlüsselworte: ,
  • Übersicht
    ArtikelnummerDIA-310
    Spezifität

    Spezies-Reaktivität

    ,

    Wirtsspezies

    Isotyp

    Klon

    Klonalität (Mono-/Polyklonal)

    Anwendung

    , , ,

    Konjugation

    Format

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    Produktlinie / Thema

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    Zweckbestimmung

    Temperatur - Lagerung

    Suchcode

    Hersteller / Marke

  • Datenblätter
  • Weitere Produktinformationen

    Reactivity:

    Der Antikörper-Klon SZ31 reagiert als erster Antikörper spezifisch mit CD31 in standardmässig Formalin-fixierten Paraffin-eingebetteten (FFPE) Mausgeweben. Klon SZ31 zeigt keine Kreureaktion mit humanem CD31.

    Die überragende Spezifität von Klon SZ31 wird in mehr als 175 Publikationen (CiteAb) bestätigt.

    Background:

    CD31, auch bekannt als PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1), wird auf der Oberfläche embryonaler und adulter Endothelzellen konstitutiv exprimiert. Sowie auf Zelloberflächen von Monozyten, Neutrophilen, Thrombozyten und verschiedener Untergruppen der T-Zellen. Zudem konnte das Molekül auf hämatopoetischen Stammzellen aus dem Knochenmark und embryonalen Stammzellen nachgewiesen werden. Cluster of Differentiation 31 (CD31) ist ein integrales Membranglykoprotein (130 kDa), welches als Mitglied der Immunglobulin-Superfamilie an der Vermittlung der Zell-Zell-Adhäsion beteiligt ist. Die von CD31 vermittelte endotheliale Zell-Zell-Interaktion, spielt hierbei eine entscheidende Rolle in der Angiogenese. Das Adhäsionsmolekül gilt als einer der wichtigsten Marker in der humanen Angiogenese, Studien zufolge kann der Nachweis von CD31 auf ein Tumorrezidiv deuten. Pathophysiologische Untersuchungen von CD31 in Maus-Modellsystemen waren bisher nur begrenzt möglich, weil Standard-FFPE Gewebe ausgeschlossen werden mussten. Der Ratten-Klon SZ31 (Anti-CD31) löst diese Probleme. Unser monoklonaler Antikörper erlaubt erstmals immunhistochemische Analysen von CD31 in Formalin-fixierten Paraffingeweben (FFPE) der Maus und überzeugt durch beeindruckende Qualität der mikroskopischen Aufnahmen.

    Immunhistochemischer Nachweis von Maus-CD31 (PECAM-1) in Formalin-fixierten, Paraffin-eingebetteten Gewebeschnitten

    Der monoklonale Antikörper Klon SZ31 reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren von:

    The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse aorta (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren der Mausaorta (FFPE Gewebe).

    aorta

    The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse aorta origin (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren der Mausaorta (FFPE Gewebe).

    aortic origin

    The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse endocardium (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren des Endokard aus der Maus (FFPE Gewebe).

    endocardium

    The monoclonal antibody clone SZ31 (DIA-310) reacts specifically with endothelial cells in vessels and capillaries of mouse small intestine (FFPE tissue). Der monoklonale Antikörper Klon SZ31 (DIA-310) reagiert spezifisch mit Endothelzellen in Gefäßen und Kapillaren des Mäuse Dünndarm (FFPE Gewebe).

    small intestine

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    small intestine

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    colon

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    brain

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    lymph nodes

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    bone marrow

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    mesenterial vessels

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    kidney

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    pancreas

    (Bilder mit freundlicher Genehmigung von Prof. Dr. Robert Klopfleisch, Institute of Pathology, Department of Veterinary Pathology, Berlin)

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    Lung

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    Muscle

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    Spinal

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    Liver

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    Adenocarcinoma

    Immunohistochemical staining (IHC) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    Adenocarcinoma

    (Bilder mit freundlicher Genehmigung von Prof. Dr. H. Stein, Institute of Pathology, Charité Campus Benjamin Franklin, Berlin)

    Western Blot-Analyse

    Immunoblot mit Zellextrakten aus Mauslunge, J558L Zellen und m-Lend Zellen. Antikörper-Klon SZ31 (DIA-310 1:500) wurde zusammen mit einem Ziege Anti-Ratte-HRP Sekundärantikörper (1:10.000) zum Nachweis von CD31 verwendet.

    Western blot detection (WB) with anti-CD31 (PECAM-1) Antibody (clone SZ31) - dianova

    Spur:

    1. J558 Zellen (CD31-), 25μg Lysat
    2. Mauslunge, 25μg Lysat
    3. blank
    4. m-Lend Zellen (CD31+), 12.5μg Lysat

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  • Bilder
    SZ31_aorta
    Aorta
    SZ31_aortic-origin
    Aortic-origin
    SZ31_bone-marrow
    Bone-Marrow
    SZ31_brain
    Brain
    SZ31_colon
    Colon
    SZ31_endocardium
    Endocardium
    SZ31_kidney
    Kidney
    SZ31_Liver
    Liver
    SZ31_Lung
    Lung
    SZ31_lymph-nodes
    Lymph-nodes
    SZ31_mesenterial-vessels
    Mesenteric vessels
    SZ31_Mouse_Ca_1
    Murine adenocarcinoma
    SZ31_Mouse_Ca_2
    Murine adenocarcinoma 2
    SZ31_Muscle
    Skeletal muscle
    SZ31_pancreas
    Pancreas
    SZ31_small-intestine-A
    Small intestine
    SZ31_small-intestine-B
    Small intestine 2
    SZ31_Spinal
    Spinal Cord
    SZ31_Western-Blot
    Western-Blot
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