Maus IgG anti-Human IgG3 (F(ab’)2) (NI 330,HP 6050)-unkonj., MinX keine

The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab’)2 portion of the IgG3 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/ml. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.