Anti-PIN-Cocktail 2 -AMACR (p504s) / Tumor Protein p63 (TP63) / CKHMW (Hu) aus Kaninchen,Maus (13H4 / 4A4 / 34ßE12) – unkonj.

The combination of AMACR (P504S), p63, and HMW CK may be extremely useful for diagnosing PIN and small focus adenocarcinoma, especially in difficult cases and cases with limited tissues. The combination of p63 and HMW CK antibodies provides a save method for the detection of basal cells, while AMACR (P504S) labels only carcinoma cells and PIN lesions. P504S (AMACR, Alpha-methylacyl-CoA racemase) is an essential enzyme in the b-oxidation of branched-chain fatty acids. High expression of AMACR protein is found in prostate adenocarcinoma but not in benign prostate tissue by immunohistochemical staining in paraffin-embedded tissue. The expression of AMACR is also detected in prostate premalignant lesions, such as prostate intraepithelial neoplasia (PIN). The p63 protein, a homologue of the tumor-suppressor p53, is highly expressed in the nuclei of basal or progenitor layer of many epithelial tissues. p63 is detected in prostate basal cells in normal prostate glands and PIN. Other markers for basal cells are high molecular cytokeratins (CK 1, 5, 10 and 14), which can be labeled with HMW CK clone 34βE12. In prostate adenocarcinoma basal cells disappear and staining with both p63 and HMW CK fails. Thus the combination of p63 and HMW CK is a useful tool as differential markers for benign prostate glands and adenocarcinoma (negative marker). 1. Human AMACR (P504S) protein, Alpha-Methylacyl CoA Racemase, 2. human p63 nuclear protein, and 3. high molecular weight cytokeratins (56-57 and 66-68 kDa).