Ziege IgG anti-Maus IgG2a (Fc)-TRITC, MinX Hu
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Übersicht
Artikelnummer GAM/IgG2a/TRITC Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klonalität (Mono-/Polyklonal) Anwendung ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (IHC)
Konjugation Keine Kreuzreaktivität (MinX) mit Format aus Antiserum, Azid-frei, IgG-Fraktion, in PBS (pH 7,2), Lyophilisat
Anwendungshinweis dies schließt eine Reaktion in empfindlicheren Nachweistechniken nicht aus, fehlende Kreuzreaktivität (MinX) zu o.g. Spezies mittels radialer Doppelimmundiffusion bestimmt, Ig-Subklassen-spezifisch
Zweckbestimmung Produktlinie / Thema Ig-spezifische Ak / Sekundärantikörper, Nordic-MUbio / Veterinärmedizin
Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
The reactivity of the antiserum is directed to the subclass IgG2a. It reacts strongly withIg2a of the allelic types Igh-1a and Igh-1b, which include BALB/C, C57BL, CBA/J, SJ/J and SM/J. When used fro the identification of IgG2a in other mouse strains the reaction may be weaker. The antiserum contains antibodies to iso and allospecific determinants. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical single and double staining for the detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, as indicating second antibody conjugate to analyze or separate human cells after reacting with monoclonal mouse antibodies in immunofluorescence microscopy, fluorescence activated cell sorting and flow cytometry, to identify and measure IgG2a in mouse serum or other body fluids.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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