Ziege F(ab’)2 anti-Human IgG+IgM (H+L)-PerCP, MinX Bo

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with both human IgG and IgM. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with immunoglobulins from other species.

Conjugate

PerCP is a fluorescent peridinin-chlorophyll-protein complex isolated from dinoflagellates. We offer the form found in Dinophyceae sp. with a molecular weight of about 35.5 kDa. It has a broad spectrum of excitation with a main peak at 482 nm, and a long Stokes shift to an emission peak at 677 nm.

PerCP, Alexa Fluor 488 (or FITC), and R-PE are excited at 488 nm with an argon laser, and thus can be used for one-, two-, and three-color analyses with single-laser flow cytometers. APC and Alexa Fluor 647 are excited at 633 nm to give a fourth color with dual-laser flow cytometers.

PerCP is also highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates.

It should be noted that the relatively high molecular weight of PerCP may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.