Ziege IgG anti-Maus IgG1 (Fc)-Alexa Fluor 647, MinX Hu,Bo,Rb
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Übersicht
Artikelnummer 115-605-205 Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klonalität (Mono-/Polyklonal) Anwendung Durchflusszytometrie (Flow Cytometry), ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe)
Konjugation Maximale Anregung Maximale Emission Keine Kreuzreaktivität (MinX) mit Verdünnung Durchflusszytometrie 1:100 – 1:800, Histo-/Zytochemie 1:100 – 1:800
Format 0,05% NaN3, 15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM PBS (pH 7,6), Lyophilisat
Anwendungshinweis Zweckbestimmung Produktlinie / Thema alte Katalognummer Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG1 but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
Conjugate
Alexa Fluor 647-conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. They are brighter than Cy5 and DyLight 650 in aqueous mounting media. Alexa Fluor 647- and APC-conjugated secondary antibodies are the best choice for flow cytometry when secondary antibodies fluorescing at these wavelengths are desired. Alexa Fluor 647 conjugates are the best choice of far red-emitting dyes for multiple-labeling detection with a confocal microscope.
A significant advantage of using Alexa Fluor 647 over lower wavelength-emitting dyes is the low autofluorescence of biological specimens in this region of the spectrum. However, because of its peak emission at 667 nm, Alexa Fluor 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Therefore, Alexa Fluor 647 is not recommended for use with conventional epifluorescent microscopes. It is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation and a far-red detector. Alexa Fluor 647 conjugates are less expensive alternatives to allophycocyanin conjugates for flow cytometry.