Anti-Acetyl-Histone H3 (Lys14) (alle) aus Kaninchen (RM130) – unkonj.
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Übersicht
Artikelnummer 31-1032-00 Spezifität Spezies-Reaktivität Wirtsspezies Isotyp Klon Klonalität (Mono-/Polyklonal) Anwendung Chromatin-Immunpräzipitation, ELISA (Enzyme Linked Immunosorbent Assay), Multiplex, Western Blot
Konjugation Format Produktlinie / Thema Zweckbestimmung Temperatur - Lagerung Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
RM130 specifically reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.
The binding specificity of RM130 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.
Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) or, using RM130 at 0.5 ug/mL.
ChIP performed on HeLa cells using H3K14ac antibody (RM130, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys14) Rabbit mAb RM130 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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Bilder
Fig-5-RM130-ICC Fig1-in-CofA-RM130 Fig2-in-CofA-RM130 Fig3-WB Fig4