Anti-Acetyl-Histone H3 (Lys9) (alle) aus Kaninchen (RM161) – unkonj.

RM161 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM161 at 0.25 ug/mL, showed a band of histone H3 acetylated at Lysine 9 in treated HeLa.

Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the capture antibody and biotinylated anti-H3K9ac (RM161, 1 ug/mL) as the detection antibody.

ChIP performed on HeLa cells using H3K9ac antibody (RM161, 5ug). Real-time PCR was performed using primers specific to the gene indicated.

Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys9) Rabbit mAb RM161 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Immunohistochemistry staining of HepG2 cells using anti-Acetyl-Histone H3 (Lys9) antibody, RM161.