Anti-Dimethyl Histone H3 (Lys79) (alle) aus Kaninchen (RM181) – unkonj.
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Übersicht
Artikelnummer 31-1065-00 Spezifität Spezies-Reaktivität Wirtsspezies Isotyp Klon Klonalität (Mono-/Polyklonal) Anwendung ELISA (Enzyme Linked Immunosorbent Assay), Multiplex, Western Blot
Konjugation Format Produktlinie / Thema Zweckbestimmung Temperatur - Lagerung Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
RM181 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with nonmodified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM181 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.
ChIP performed on HeLa cells using H3K79me2 antibody (RM181, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
Immunohistochemistry staining of HepG2 cells using Anti-Dimethyl-Histone H3 (Lys79) antibody, RM181.
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Bilder
fig1-RM181 fig2-RM181 fig3-RM181 fig4-HepG2_H3K79M2_IHC