Anti-Acetyl-Histone H3 (Lys56) (alle) aus Kaninchen (RM179) – unkonj.
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Übersicht
Artikelnummer 31-1078-00 Spezifität Spezies-Reaktivität Wirtsspezies Isotyp Klon Klonalität (Mono-/Polyklonal) Anwendung ELISA (Enzyme Linked Immunosorbent Assay), Multiplex, Western Blot
Konjugation Format Anwendungshinweis No cross reactivity with other acetylated Lysines in histone H3., Reacts to Histone H3 acetylated at Lysine 56 (K56ac)
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Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant histone H3.3, using RM179 at 1 µg/mL, showed a band of histone H3 acetylated at Lysine 56 in treated HeLa.
RM179 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys56) Rabbit mAb RM179 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Immunohistochemistry staining of HepG2 cells using Anti-Acetyl-Histone H3 (Lys56) antibody, RM179.
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Bilder
Fig-1_RM179 Fig-3_RM179_ICC Fig2 fig4-HepG2_K56Ac_IHC