Anti-His Epitop-Tag (rek) aus Maus (13/45/31) – unkonj.

The mouse monoclonal anti-(His)6-tag antibody, clone 13/45/31-2 (H. Zentgraf/DKFZ Heidelberg, Germany), specifically detects any kind of histidine-tagged proteins in cells and complex cellular lysates. This monoclonal antibody specifically reacts with recombinant proteins containing an epitope of at least 6 histidine residues, located at the N-terminus, C-terminus or internally. A higher number of histidine residues leads to an increased binding affinity of the antibody, e.g. an expression construct with 10 histidine residues increases the affinity about 10- to 20-fold. Additional flanking amino acids are not required for antibody binding, therefore enabling the choice of many different expression vectors on the only condition that the TAG-epitope is sterically available.

The Antibody clone 13/45/31-2 /13/45/31 is with more than 100 citations one of the most cited His-Tag antibodies world wide.