Anti-IDH1 (Hu) aus Ratte (W09) – unkonj.

Reactivity

Isocitrate dehydrogenase (IDH) is an important enzyme in the citric acid cycle and catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate and CO2 while converting NAD+ to NADH. Heterozygous point mutations of IDH1 codon 132 are frequent in World Health Organization (WHO) grade II and III gliomas. IDH1 R132H mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors. Mouse antibody clone H09 (product number #DIA-H09) reacts specifically with the isocitrate dehydrogenase 1 (IDH1) R132H point mutation in tissue sections from formalin-fixed brain tumor specimens. Therefore this antibody is highly useful for tumor classification. Rat antibody clone W09 detects both wild type (wt) and point mutated IDH1 in glioblastoma (anti-pan-IDH1) and serves as control for the IDH1 point mutation specific mouse antibody clone H09. Moreover, Tan et al. (Mol Cell Proteomics, 2011) have identified IDH1 as a potential diagnostic and prognostic biomarker for Non-small-cell Lung Cancer (NSCLC). These findings suggest that anti-IDH1wt antibody could be used as a histochemical biomarker for prognosis prediction of NSCLC.

Immunohistochemistry of human IDH1 R132H and human IDH1 wild-type in formalin-fixed paraffinembedded glioblastoma.

A: No reaction of IDH1 R132H mutation specific antibody clone H09 with glioblastoma (200x)
B: Strong reaction of anti-pan-IDH1 specific antibody clone W09 with the same glioblastoma (200x)
C: No reaction of IDH1 R132H mutation specific antibody clone H09 with glioblastoma (400x)
D: Strong reaction of anti-pan-IDH1 specific antibody clone W09 with the same glioblastoma (400x)

A

B

C

D

(pictures courtesy of Prof. Dr. med. Andreas von Deimling, Department of Neuropathology, University Heidelberg / Clinical Cooperation Unit Neuropathology, German Cancer Research Center (DKFZ), Heidelberg, Germany)

References

• Capper D, Weissert S, Balss J, Habel A, Meyer J, Jäger D, Ackermann U, Tessmer C, Korshunov A, Zentgraf H, Hartmann C, von Deimling A. Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. Brain Pathol. 20(1): 245-254, 2010

Related References

• Tan F, Jiang Y, Sun N, Chen Z, Lv Y, Shao K, Li N, Qiu B, Gao Y, Li B, Tan X, Zhou F, Wang Z, Ding D, Wang J, Sun J,Hang J, Feng X, Shi S, He F, He J.. Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non small-cell Lung Cancer by Proteomic Analysis. Mol Cell Proteomics 2011 Nov 7. (Epub ahead of print)