Ziege IgG anti-Kaninchen IgG (Fc)-Biotin, MinX keine
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Übersicht
Artikelnummer GAR/IgG(Fc)/Bio Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klonalität (Mono-/Polyklonal) Anwendung Andere, ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunhistochemie (Paraffingewebe), Western Blot
Konjugation Keine Kreuzreaktivität (MinX) mit Format aus Antiserum, Azid-frei, IgG-Fraktion, in PBS (pH 7,2), Lyophilisat
Anwendungshinweis geeignet zum Nachweis von 25kD-Proteinen im Western Blot nach Immunpräzipitation, reagiert nicht mit IgG, F(ab')2-Fragment, reagiert nicht mit leichten Immunglobulin-Ketten
Zweckbestimmung Produktlinie / Thema Ig-spezifische Ak / Sekundärantikörper, Nordic-MUbio / Veterinärmedizin
Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical use for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using an reference antibody of goat origin known to be of the IgG isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG in rabbit serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user is choice has to be used.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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