Maus IgG anti-Human IgG3 (F(ab’)2) (NI 330,HP 6050)-HRPO, MinX keine

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Übersicht
Artikelnummer MAHu/IgG3/PO Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klon Klonalität (Mono-/Polyklonal) Anwendung Andere, ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunhistochemie (Paraffingewebe), Western Blot
Konjugation Keine Kreuzreaktivität (MinX) mit Format aus Ascites, gereinigter Antikörper, in PBS (pH 7,2), Lyophilisat
Anwendungshinweis Zweckbestimmung Produktlinie / Thema Hersteller / Marke - Datenblätter und Downloads
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Weitere Produktinformationen
The reactivity of the antiserum is restricted to the subclass specific determinant on the F(ab’)2 portion of the IgG3 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.
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