Maus IgG anti-Human IgG (Fc) (NI 335 + NI 343)-Biotin, MinX keine

The reactivity of the antiserum is restricted to isotype specific determinants on the Fc portion of the IgG molecule. The antiserum reacts with the 4 subclasses of human IgG, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer is choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 250, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards.

Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 3.2. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.