Kaninchen IgG anti-Schwein IgG (Fc)-HRPO, MinX keine
-
Übersicht
Artikelnummer RASw/IgG(Fc)/PO Wirtsspezies IgG-Form Spezies-Reaktivität Spezifität Isotyp Klonalität (Mono-/Polyklonal) Anwendung Andere, ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunhistochemie (Paraffingewebe), Western Blot
Konjugation Keine Kreuzreaktivität (MinX) mit Format aus Antiserum, Azid-frei, IgG-Fraktion, in PBS (pH 7,2), Lyophilisat
Zweckbestimmung Produktlinie / Thema Nordic-MUbio / Sekundärantikörper, Nordic-MUbio / Veterinärmedizin
Hersteller / Marke - Datenblätter und Downloads
-
Weitere Produktinformationen
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal swine plasma and serum, a single characteristic precipitin line is obtained, which shows a reaction of identity with the precipitin line obtained with purified IgG. It does not react with any non-Ig protein in swine serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Kontakt